Evaluating the Influence of Agro-Environmental Measures on Honey Bees Based on Genetic Pollen Analysis With Nanopore Sequencing





Intensive land use is one of the main drivers contributing to the loss of pollinating insects due to the distribution of pesticides and the reduction of flower diversity. This work analyzes honey bee pollen samples taken from areas of different agricultural intensity. The aim is to classify the plant species contained in the pollen samples using DNA barcodes and nanopore sequencing in order to evaluate the influence of different agro-environmental measures on the foraging behavior of honey bees. First results concern the DNA extraction and the identification of universal primer sequences in preparation for sequencing with the MinION Mk1c from Oxford Nanopore Technologies.


Vanbergen, A. J. and The Insect Pollinators Initiative (2013): Threats to an ecosystem service: Pressures on pollinators. Frontiers in Ecology and the Environment 11(5): 251–259.

Klein, A.-M. et al. (2007): Importance of pollinators in changing landscapes for world crops. Proceedings of the Royal Society B. Biological sciences, 274: 303–313.

Haaland, C.; Naisbit, R. E. and Bersier, L.-F. (2011): Sown wildflower strips for insect conservation: A review. Insect Conservation and Diversity, 4: 60–80.

Pornon, A. et al. (2016): Using metabarcoding to reveal and quantify plant-pollinator interactions. Scientific reports, 6(27282): 1–12.

Leidenfrost, R. M. et al. (2020): Analyzing the dietary diary of bumble bee. Frontiers in plant science, 11(287): 1–9.

Westphal, C.; Hass, A. and Paxton, R. (2021): ComBee: Kombinierte Agrarumwelt-maßnahmen, Bienendiversität und Gesundheitszustand von Wild- und Honigbienen. https://www.uni goettingen.de/de/646422.html [05.05.2022].

Fišer Pečnikar, Ž. and Buzan, E. V. (2014): 20 years since the introduction of DNA barcoding: From theory to application. Journal of applied genetics, 55: 43–52.

Li, F.-S. et al. (2019): The molecular structure of plant sporopollenin. Nature plants, 5: 41–46.

Hawkins, J. et al. (2015): Using DNA metabarcoding to identify the floral composition of honey: A new tool for investigating honey bee foraging preferences. PloS one, 10(8): 1–20.

Bell, K. L. et al. (2017): Applying pollen DNA metabarcoding to the study of plant-pollinator interactions. Applications in plant sciences, 5(6): 1–10.

Richardson, R. T. et al. (2021): Application of plant metabarcoding to identify diverse honeybee pollen forage along an urban-agricultural gradient. Molecular ecology, 30: 310–323.



How to Cite

Pannicke, B., Prudnikow, L., & Wünschiers, R. (2022). Evaluating the Influence of Agro-Environmental Measures on Honey Bees Based on Genetic Pollen Analysis With Nanopore Sequencing. Open Conference Proceedings, 2, 201–203. https://doi.org/10.52825/ocp.v2i.166



Beiträge zur / Contributions to the 22. Nachwuchswissenschaftler*innenkonferenz (NWK)